Enzymatic remodeling of heparan sulfate: a therapeutic strategy for systemic and localized amyloidoses?

Enzymatic remodeling of heparan sulfate: a therapeutic strategy for systemic and localized amyloidoses?

Enzymatic redesigning of biologically active heparan sulfate.

Heparan sulfate carries a wide range of biological activities, regulating blood coagulation, cell differentiation, and inflammatory responses. The sulfation patterns of the polysaccharide are essential for the biological activities. In this study, we report an enzymatic method for the sulfation of multimilligram amounts of heparan sulfate with specific functions using immobilized sulfotransfera...

Combinatorial enzymatic synthesis of heparan sulfate.

Escherichia coli K5 heparosan was enzymatically modified by Chen and colleagues to construct a library of heparan sulfate polysaccharides for evaluation, leading to the discovery that a 2-O-sulfoiduronic acid residue is not essential for antithrombin-mediated anticoagulant activity in larger oligosaccharide and polysaccharide structures.

Physiological, Pathophysiological and Therapeutic Roles of Heparin and Heparan Sulfate

Heparin and heparan sulfate Au3 belong to the glycosaminoglycan (GAG) family of carbohydrates. They are linear acidic complex polysaccharides found on the cell surface and in the extracellular matrix (1). Heparin and heparan sulfate GAGs are biosynthesized as proteoglycans (PGs) with multiple GAG chains linked to a variety of core proteins. Heparin PGs are found exclusively in the granules of s...

Orthogonal sulfation strategy for synthetic heparan sulfate ligands.

[reaction: see text] An orthogonal sulfation strategy involving six different protecting groups has been developed for generating sulfated carbohydrate libraries based on heparan. Chemoselective cleavage conditions (optimized for a heparan disaccharide) can be performed in the presence of sulfate esters as well as the remaining protecting groups.